منابع مشابه
Multiplex Flow Assays
Lateral flow or dipstick assays (e.g., home pregnancy tests), where an analyte solution is drawn through a porous membrane and is detected by localization onto a capture probe residing at a specific site on the flow strip, are the most commonly and extensively used type of diagnostic assay. However, after over 30 years of use, these assays are constrained to measuring one or a few analytes at a...
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Multiplex polymerase chain reaction (PCR) refers to the simultaneous amplification of multiple regions of deoxyribonucleic acid (DNA) using PCR. Commercial short tandem repeat (STR) assays that can coamplify as many as 16 different loci have become widely used in forensic DNA typing. This chapter will focus on some of the aspects of constructing robust STR multiplex assays, including careful de...
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The ability to multiplex PCR by probe color and melting temperature (T(m)) greatly expands the power of real-time analysis. Simple hybridization probes with only a single fluorescent dye can be used for quantification and allele typing. Different probes are labeled with dyes that have unique emission spectra. Spectral data are collected with discrete optics or dispersed onto an array for detect...
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Panomics has an aggressive program for the development of a broad range of assays for the Luminex platform and similar instruments based on the xMAP technology. Currently we have hundreds of RNA targets available in 3–30 plex assays using our using our QuantiGene Plex Reagent Systems, with new targets being added weekly. Our Procarta® Human, Mouse and Rat Assays can quantitatively measure cytok...
متن کاملLateral flow assays
Lateral flow assays (LFAs) are the technology behind low-cost, simple, rapid and portable detection devices popular in biomedicine, agriculture, food and environmental sciences. This review presents an overview of the principle of the method and the critical components of the assay, focusing on lateral flow immunoassays. This type of assay has recently attracted considerable interest because of...
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ژورنال
عنوان ژورنال: ACS Omega
سال: 2016
ISSN: 2470-1343,2470-1343
DOI: 10.1021/acsomega.6b00188